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+WORD: After the coupling of the primary C-terminal amino acid of all peptides to the amino group of the membrane, the amino teams between the spots and also a few of the amino teams within the spot areas don't kind bonds with the amino acids. OBSERVE: This avoids the coupling of amino acids within the additional cycles with the membrane as an alternative of the rising peptide chain. Be certain that all the purification buffers are at four °C and carry out the protein purification in the cold room to keep away from protein denaturation.
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